snu5 cell lines  (ATCC)

Journal: Cell Death Discovery

Article Title: Gastric cancer cell-derived extracellular vesicles elevate E2F7 expression and activate the MAPK/ERK signaling to promote peritoneal metastasis through the delivery of SNHG12

doi: 10.1038/s41420-022-00925-6

Figure Lengend Snippet: A The expression of SNHG12, LINC00665, and TTN-AS1 in GC and adjacent normal tissues examined using RT-qPCR ( n = 69). B The relative level of SNHG12 in GC tissues from patients with GC of different clinical stages (I ( n = 22); II and III ( n = 47)) determined using RT-qPCR. C SNHG12 expression pattern in GC tissues with peritoneal metastasis ( n = 31) and GC tissues without peritoneal metastasis ( n = 38) from patients with GC detected by RT-qPCR. D The correlation between SNHG12 expression pattern and the overall survival of patients with GC analyzed by Kaplan–Meier survival curve. E The expression pattern of SNHG12 in serum-derived EVs in 10 healthy volunteers, 38 patients with non-metastatic GC, and 31 patients with metastatic GC determined by RT-qPCR. F SNHG12 expression pattern in normal human gastric mucosal cells GES-1 and GC cell lines HGC-27, NCI-N87, SNU-1, and SNU5 measured using RT-qPCR. G SNHG12 expression pattern in EVs isolated from each group of GC cells evaluated by RT-qPCR. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001. The experiment was conducted three times independently.

Article Snippet: The GC cell line SNU5 and HEK-293T were provided by ATCC (Manassas, VA, USA).

Techniques: Expressing, Quantitative RT-PCR, Derivative Assay, Isolation